AsperGenius®: a multiplex Real Time PCR assay for the detection of Aspergillus fumigatus and identification of azole resistance markers



AsperGenius® is a multiplex real-time PCR assay developed by PathoNostics. It rapidly diagnoses Aspergillus infections and simultaneously identifies azole resistance. Within 2,5 hours, detection and characterization is accomplished in lower respiratory tract samples of Aspergillus infected patients. AsperGenius® allows for timely, targeted antifungal treatment resulting in reduced toxic side effects and improved treatment outcome in these patients.

The AsperGenius® multiplex PCR assay is suitable for real-time PCR instruments using melting curve analysis and has been validated on LightCycler 480 (Roche), Rotor-Gene 6000 (Corbett) and Rotor-Gene Q (Qiagen).


AsperGenius® Species multiplex (PN-001)

  • 50 reactions
  • Detection and differentiation of Aspergillus species

AsperGenius® Resistance multiplex (PN-002)

  • 50 reactions
  • Detection and differentiation of Aspergillus species
  • Identification of 4 azole resistance markers in Aspergillus fumigatus


Species multiplex  

  • Aspergillus fumigatus    
  • Aspergillus terreus
  • Aspergillus species
  • Internal Amplification Control (IAC)

Resistance multiplex

  • L98H
  • Tandem repeat 34
  • T289A
  • Y121F

Diagnostic Specimens

  • Bronchoalveolar lavage (BAL) samples from hematology patients
  • Bronchoalveolar lavage (BAL) samples from intensive care unit patients


  • Validated on fungal cultures
  • Validated on clinical samples (BAL)
  • CE-IVD marked

Features & benefits

  • Direct detection on clinical samples
  • Identification of the most prevalent triazole resistance mutations
  • Diagnosis within 2,5 hours after nucleic acid extraction
  • Internal Amplification Control (IAC) included
  • Positive controls included
  • Interpretation software available


AsperGenius flyer (pdf, 2 MB)