PneumoGenius®: a multiplex real-time PCR assay for the detection and quantification of Pneumocystis jirovecii and DHPS mutations.



PneumoGenius® is a multiplex real-time PCR assay that detects Pneumocystis jirovecii in respiratory tract samples within 2.5 hours. Pneumocystis jirovecii is a common cause of life-threatening pneumonia in immunocompromised patients. Fast diagnosis and the initiation of appropriate therapy may lead to improved outcome and survival. The PneumoGenius® assay also determines the presence of 2 mutations that are associated with failure of treatment using sulfa-drugs. These mutations are located on codons 55 and 57 of the multi-domain FAS gene, encoding the enzyme dihydropteroate synthase (DHPS).

The PneumoGenius® multiplex PCR assay is suitable for real-time PCR instruments using melting curve analysis, and has been validated on LightCycler 480 (Roche) and Rotor-Gene Q (Qiagen), CFX96™ (Bio-Rad) and the Mic qPCR (BMS).


PneumoGenius® multiplex kit
(PN-600) -  25 reactions

  • Detection of Pneumocystis jirovecii
  • Quantification of Pneumocystis jirovecii
  • Identification of mutations in DHPS


  • Pneumocystis jirovecii mtLSU    
  • Pneumocystis jirovecii DHPS domain of the FAS gene (codons 55 and 57)
  • Internal Control

Diagnostic Specimens

  • Bronchoalveolar lavage (BAL) samples


  • Validated on clinical samples (BAL)
  • Validated on PCP EQA programmes (QCMD)
  • CE-IVD marked

Features & benefits

  • Direct detection in BAL samples
  • Identification of two prevalent point mutations in the DHPS domain of the FAS gene
  • Diagnosis within 2,5 hours after nucleic acid extraction
  • Internal Control included
  • Positive control included
  • Including 4 quantification standards


PneumoGenius flyer (pdf)